BIO 212L 01Agenda:

Week

Introduction

Molecular genetics has opened the door to biotechnology. This week we continue our discovery by carrying out the digestion of $LaTeX: \lambda$ -phage with restriction enzymes and then running it on an agarose gel vie electrophoresis.

This is a demanding lab and it will require that you come prepared to lab. There are literally three parts to the investigation that must be done within two hours and 50 minutes. Please prepare yourselves by reading the student manual and writing a well-prepared pre-lab in your laboratory journal.

Module Learning Outcomes

Understand the use of restriction enzymes as biotechnology tools
Become familiar with principles and techniques of agarose gel electrophoresis
Generate a standard curve from a series of DNA size standards
Estimate DNA fragments sizes from agarose gel data

Lesson 01:

Aliquot reagents, such as restriction enzymes, buffers, and lambda DNA (optional)
Pour agarose gels

Lesson 02:

Prepare HindIII lambda digest (DNA marker) and aliquot (optional)
Aliquot sample DNA loading dye (optional)
Prepare the electrophoresis chamber
Dilute Fast Blast to 100x (for quick staining)

Learning Activities

Activities

Reading: Lambda Phage Restriction Student Manual.pdf
Lab: Quick Guide For Restriction Digestion and Analysis.pdf

Assignments:

Assignment:
- Restriction Digestion and Analysis of Lambda DNA Data Sheet.docx
- Lambda phage Data Sheet.xlsx

Resources

Textbook: Lambda Phage Restriction Student Manual.pdf

Presentation:

Video:

Weekly Time Expectations

All times are approximate. Some activities may take less or more time. Please try to plan accordingly.

Reading: 30 minutes
Lab: 2 hours 45 minutes
Assignment: 45 minutes
Resources:
- Restriction Digestion and Analysis of Lambda DNA Data Sheet.docx
- Lambda phage Data Sheet.xlsx